unc5b antibody Search Results


unc5b  (Bioss)
90
Bioss unc5b
Primer sequences of netrin-1 receptors
Unc5b, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/UNC5B+UNC5H2+Polyclonal+Antibody/pmc07859736-147-16-21
Average 90 stars, based on 1 article reviews
unc5b - by Bioz Stars, 2026-09
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unc5h2/unc5b antibody  (Bio-Techne corporation)
90
Bio-Techne corporation unc5h2/unc5b antibody
Primer sequences of netrin-1 receptors
Unc5h2/Unc5b Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/UNC5H2%2FUNC5B+Antibody/bio-techne+corporation___nbp2-26090
Average 90 stars, based on 1 article reviews
unc5h2/unc5b antibody - by Bioz Stars, 2026-09
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unc5b  (R&D Systems)
92
R&D Systems unc5b
A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + <t>Sh‐UNC5B)</t> injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.
Unc5b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/Rat+UNC5H2%2FUNC5B+Antibody/pmc07849168-259-37-38
Average 92 stars, based on 1 article reviews
unc5b - by Bioz Stars, 2026-09
92/100 stars
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goat anti unc5b antibody  (R&D Systems)
99
R&D Systems goat anti unc5b antibody
A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + <t>Sh‐UNC5B)</t> injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.
Goat Anti Unc5b Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/Rat+UNC5H2%2FUNC5B+Antibody/pm30479344-364-11-15
Average 99 stars, based on 1 article reviews
goat anti unc5b antibody - by Bioz Stars, 2026-09
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unc5b goat af1006 r d systems  (R&D Systems)
94
R&D Systems unc5b goat af1006 r d systems
A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + <t>Sh‐UNC5B)</t> injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.
Unc5b Goat Af1006 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/Rat+UNC5H2%2FUNC5B+Antibody/pm41703986-145-15-18
Average 94 stars, based on 1 article reviews
unc5b goat af1006 r d systems - by Bioz Stars, 2026-09
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goat polyclonal anti unc5b  (R&D Systems)
90
R&D Systems goat polyclonal anti unc5b
A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + <t>Sh‐UNC5B)</t> injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.
Goat Polyclonal Anti Unc5b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/UNC5H2%2FUNC5B+Antibody/bio_rxiv__2020__08__06__234906-208-33-36
Average 90 stars, based on 1 article reviews
goat polyclonal anti unc5b - by Bioz Stars, 2026-09
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anti-human unc5b antibody  (Genentech inc)
90
Genentech inc anti-human unc5b antibody
A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + <t>Sh‐UNC5B)</t> injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.
Anti Human Unc5b Antibody, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/anti+human+unc5b+antibody/pmc03096761-78-17-23
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rabbit anti unc5b  (R&D Systems)
93
R&D Systems rabbit anti unc5b
A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + <t>Sh‐UNC5B)</t> injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.
Rabbit Anti Unc5b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/Rat+UNC5H2%2FUNC5B+Antibody/pm35247371-80-29-32
Average 93 stars, based on 1 article reviews
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unc5h2/unc5b antibody (1a9)  (Bio-Techne corporation)
90
Bio-Techne corporation unc5h2/unc5b antibody (1a9)
A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + <t>Sh‐UNC5B)</t> injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.
Unc5h2/Unc5b Antibody (1a9), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+antibody/UNC5H2%2FUNC5B+Antibody+(1A9)/bio-techne+corporation___h00219699-m01
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unc5b antibody (fitc)  (biorbyt)
N/A
Rabbit polyclonal antibody against UNC5B conjugated to FITC Isotype Note: IgG Host Note: Rabbit Conjugation Note: FITC Reactivity Note: Human Application Note: ELISA
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unc5b rabbit polyclonal antibody  (OriGene)
N/A
UNC5B rabbit polyclonal antibody
   Buy from Supplier
goat anti-unc5b (mouse, rat) antibody  (Angio-Proteomie)
N/A
   Buy from Supplier

Image Search Results


Primer sequences of netrin-1 receptors

Journal: Annals of Translational Medicine

Article Title: Macrophage-derived netrin-1 contributes to endometriosis-associated pain

doi: 10.21037/atm-20-2161

Figure Lengend Snippet: Primer sequences of netrin-1 receptors

Article Snippet: The specific antibodies of netrin-1 (1:50, ab122903, goat polyclonal, Abcam), DCC (1:300, bs-0592R, rabbit polyclonal, Bioss), UNC5B (1:300, bs-11492R, rabbit polyclonal, Bioss) and A2BAR (1:1,000, PA5-72850, rabbit polyclonal, Invitrogen) were used to access the expression levels and localization of netrin-1 and its receptors in endometrial tissues.

Techniques:

Expression levels of netrin-1 receptors in endometrial tissues. The mRNA expression levels of DCC (A), neogenin (B), UNC5A (C), UNC5B (D), UNC5C (E), UNC5D (F), DSCAM (G), CD146 (H) and A2BAR (I) in endometriotic lesions and eutopic endometria from women with or without endometriosis were tested using RT-qRCR. Error bars show mean ± SEM. * P<0.05; ** P<0.01; *** P<0.0001 (one-way ANOVA and Student’s t-test). SEM, standard error of the mean.

Journal: Annals of Translational Medicine

Article Title: Macrophage-derived netrin-1 contributes to endometriosis-associated pain

doi: 10.21037/atm-20-2161

Figure Lengend Snippet: Expression levels of netrin-1 receptors in endometrial tissues. The mRNA expression levels of DCC (A), neogenin (B), UNC5A (C), UNC5B (D), UNC5C (E), UNC5D (F), DSCAM (G), CD146 (H) and A2BAR (I) in endometriotic lesions and eutopic endometria from women with or without endometriosis were tested using RT-qRCR. Error bars show mean ± SEM. * P<0.05; ** P<0.01; *** P<0.0001 (one-way ANOVA and Student’s t-test). SEM, standard error of the mean.

Article Snippet: The specific antibodies of netrin-1 (1:50, ab122903, goat polyclonal, Abcam), DCC (1:300, bs-0592R, rabbit polyclonal, Bioss), UNC5B (1:300, bs-11492R, rabbit polyclonal, Bioss) and A2BAR (1:1,000, PA5-72850, rabbit polyclonal, Invitrogen) were used to access the expression levels and localization of netrin-1 and its receptors in endometrial tissues.

Techniques: Expressing

DCC, UNC5B and A2BAR immunoreactive staining in endometrial tissues. The locations and expression levels of DCC (A,B,C), UNC5B (D,E,F) and A2BAR (G,H,I) in endometriotic lesions (A,D,G) and eutopic endometrium from women with (B,E,H) and without (C,F,I) endometriosis were measured using immunohistochemistry analysis.

Journal: Annals of Translational Medicine

Article Title: Macrophage-derived netrin-1 contributes to endometriosis-associated pain

doi: 10.21037/atm-20-2161

Figure Lengend Snippet: DCC, UNC5B and A2BAR immunoreactive staining in endometrial tissues. The locations and expression levels of DCC (A,B,C), UNC5B (D,E,F) and A2BAR (G,H,I) in endometriotic lesions (A,D,G) and eutopic endometrium from women with (B,E,H) and without (C,F,I) endometriosis were measured using immunohistochemistry analysis.

Article Snippet: The specific antibodies of netrin-1 (1:50, ab122903, goat polyclonal, Abcam), DCC (1:300, bs-0592R, rabbit polyclonal, Bioss), UNC5B (1:300, bs-11492R, rabbit polyclonal, Bioss) and A2BAR (1:1,000, PA5-72850, rabbit polyclonal, Invitrogen) were used to access the expression levels and localization of netrin-1 and its receptors in endometrial tissues.

Techniques: Staining, Expressing, Immunohistochemistry

A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + Sh‐UNC5B) injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.

Journal: The EMBO Journal

Article Title: Netrin‐1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson’s disease features

doi: 10.15252/embj.2020105537

Figure Lengend Snippet: A Representative images of TH + dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6‐GFP); Cre (AAV6‐GFP Cre); sh DCC (AAV6‐GFP Cre + sh DCC); and shUNC5B (AAV6 GFP Cre + Sh‐UNC5B) injection into the SN of Netrin‐1 fl/fl mice. (Scale bar, 2,000 µm). B Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin‐1 fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t ‐test. N = 3 each group. Tukey post hoc analysis after one‐way ANOVA * P < 0.05, ** P < 0.01, N.S., not significant. C DCC depletion mitigates netrin‐1 depletion‐induced apoptosis in Netrin‐1 fl/fl mice. TH‐positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5‐white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH‐positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; **P < 0.01. D Immunoblot of SN lysates (upper panel) and corresponding quantification (bottom panel). Quantification of band intensity (bottom). Mouse N = 2. N = 3 independent immunoblot analysis. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t ‐test. *P < 0.05; P** < 0.01. E Motor behavior tests at 6 weeks after treatment. N = 8 animals/group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. *P < 0.05; N.S., not significant. Source data are available online for this figure.

Article Snippet: Primary antibodies to the following targets were used: alpha‐synuclein FL (Santa Cruz, Cat# SC69977) alpha‐synuclein pS129 (LS bio, Cat# LS‐C380861‐1); Tyrosine Hydroxylase (Santa Cruz, SC‐25269; Abcam, Cat# ab112); Netrin‐1(Santa Cruz, Cat# SC20786 or SC‐293197; Abcam, Cat# ab126729); UNC5B (R&D systems, Cat#MAB1006); DCC (Santa Cruz, Cat# SC515834); and cleaved caspase‐3 (Cell signaling Cat# 9661).

Techniques: Staining, Immunofluorescence, Injection, TUNEL Assay, Comparison, Western Blot

A Netrin‐1 gene expression profiling by array of substantiae nigrae from PD and non‐PD (Control) patients using the GEO dataset GSE7621 that has a total of 25 samples ( n = 9 control and n = 16 PD cases). Unpaired t ‐test, *** P < 0.0005, Mean ± SD are shown. B Immunoblot of netrin‐1, DCC, UNC5B, active caspase‐3, alpha‐synuclein FL, alpha‐synuclein pS129, and tyrosine hydroxylase (TH) levels in age‐matched controls ( n = 3) vs Parkinson’s disease patients brain samples ( n = 3) (left panel). Band quantification bar graph (right panel). Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. ** P < 0.01. N = 3 replicates. Source data are available online for this figure.

Journal: The EMBO Journal

Article Title: Netrin‐1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson’s disease features

doi: 10.15252/embj.2020105537

Figure Lengend Snippet: A Netrin‐1 gene expression profiling by array of substantiae nigrae from PD and non‐PD (Control) patients using the GEO dataset GSE7621 that has a total of 25 samples ( n = 9 control and n = 16 PD cases). Unpaired t ‐test, *** P < 0.0005, Mean ± SD are shown. B Immunoblot of netrin‐1, DCC, UNC5B, active caspase‐3, alpha‐synuclein FL, alpha‐synuclein pS129, and tyrosine hydroxylase (TH) levels in age‐matched controls ( n = 3) vs Parkinson’s disease patients brain samples ( n = 3) (left panel). Band quantification bar graph (right panel). Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one‐way ANOVA followed by post hoc Tukey test for multiple group comparison. ** P < 0.01. N = 3 replicates. Source data are available online for this figure.

Article Snippet: Primary antibodies to the following targets were used: alpha‐synuclein FL (Santa Cruz, Cat# SC69977) alpha‐synuclein pS129 (LS bio, Cat# LS‐C380861‐1); Tyrosine Hydroxylase (Santa Cruz, SC‐25269; Abcam, Cat# ab112); Netrin‐1(Santa Cruz, Cat# SC20786 or SC‐293197; Abcam, Cat# ab126729); UNC5B (R&D systems, Cat#MAB1006); DCC (Santa Cruz, Cat# SC515834); and cleaved caspase‐3 (Cell signaling Cat# 9661).

Techniques: Gene Expression, Control, Western Blot, Comparison

Journal: The EMBO Journal

Article Title: Netrin‐1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson’s disease features

doi: 10.15252/embj.2020105537

Figure Lengend Snippet:

Article Snippet: Primary antibodies to the following targets were used: alpha‐synuclein FL (Santa Cruz, Cat# SC69977) alpha‐synuclein pS129 (LS bio, Cat# LS‐C380861‐1); Tyrosine Hydroxylase (Santa Cruz, SC‐25269; Abcam, Cat# ab112); Netrin‐1(Santa Cruz, Cat# SC20786 or SC‐293197; Abcam, Cat# ab126729); UNC5B (R&D systems, Cat#MAB1006); DCC (Santa Cruz, Cat# SC515834); and cleaved caspase‐3 (Cell signaling Cat# 9661).

Techniques: